MOLECULAR IDENTIFICATION OF Pepper yellow leaf curl Indonesia virus ON CHILI PEPPER IN NUSA PENIDA ISLAND

Molecular identification of Pepper yellow leaf curl Indonesia virus on chili pepper in Nusa Penida Island. (PYLCV) has been reported as caused disease Bali Island since early 2012. Dominant symptoms PYLCV infection were yellowing, curl, mosaic, and mottle. Bemisia tabaci, known to vector the case disease. Observations 2020 showed such disease, however, not studied. was conducted using polymerase chain reaction sequence analysis. Data collected this study incidence. The results that dominant by from Universal DNA fragments 912 bp successfully amplified 50 samples Begomovirus degenerate primers SPG 1 (5’-CCCCKGTGCGWRAATCCAT-3’) 2 (5’ATCCVAA YWTYCAGGGAGCT-3’). Sequence analysis isolate a with 98–100% homology several reference isolates.